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Ready, Set, E.coli!!!

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 Ready, Set, E.coli!!! Continue our AMR journey with us this week as we inoculate and further test our samples that were previously collected Day 1: Inoculation onto EMB plates Eosin-Methylene Blue Agar (EMB) is a selective and differential media used for gram negative bacterial identification as they ferment lactose causing a color change of the media to either a metallic green or black centered colony. An example of such bacteria is E.coli.  The plates were inoculated using 3 methods. Method 1: Centrifugation Method 2: Direct Plating  Method 3: Spread Plating   Day 2: Inoculation onto Blood Agar Blood agar medium allows for the fastidious growth of bacteria as it is very nutrient rich. This was chosen to grow the black centered colonies separately from the Metallic green colonies.  Day 3: Biochemical testing  Biochemical testing is a crucial step in identifying bacteria and determining its characteristics, allowing for proper diagnosis and treatment....
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The Resistance is Out There. . .

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Come collect some samples with us! What's the scoop with AMR and public wastewater? Antimicrobial resistance (AMR) is one of the most pressing global health threats of our time. It occurs when bacteria, like Escherichia coli , develop the ability to survive treatments with antibiotics that would normally kill them, making common infections harder to treat and increasing the risk of disease spread and severe illness. In areas where urban, agricultural, and industrial activities often intersect, runoff and floodwaters may serve as silent reservoirs for antibiotic-resistant bacteria. In this project, we explore the presence of AMR E. coli in water from various high-risk environments and assess the potential exposure risks to nearby human populations through antibiotic susceptibility testing. Come along with us! Here’s what you’ll need:  Gloves Sterile sample cups Ziploc bags Scooping pole Cooler for samples 70% isopropyl alcohol Permanent marker for labelling  Location Selecti...
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