Ready, Set, E.coli!!!
Ready, Set, E.coli!!!
Continue our AMR journey with us this week as we inoculate and further test our samples that were previously collected
Day 1: Inoculation onto EMB plates
Eosin-Methylene Blue Agar (EMB) is a selective and differential media used for gram negative bacterial identification as they ferment lactose causing a color change of the media to either a metallic green or black centered colony. An example of such bacteria is E.coli.
The plates were inoculated using 3 methods.
Method 1: Centrifugation
Method 2: Direct Plating
Method 3: Spread Plating
Day 2: Inoculation onto Blood Agar
Blood agar medium allows for the fastidious growth of bacteria as it is very nutrient rich. This was chosen to grow the black centered colonies separately from the Metallic green colonies.
Day 3: Biochemical testing
Biochemical testing is a crucial step in identifying bacteria and determining its characteristics, allowing for proper diagnosis and treatment.
Our specific biological tests included: Citrate, Urea, SIM (Sulfide Indole Motility), TSI (Triple Sugar Iron), LIA(Lysine Iron Agar) and MR (Methyl Red)
Day 4: Antibiotic Testing
1. A BHI (Brain Heart Infusion broth) was mixed with one of our inoculated colonies to the same translucency as the McFarland’s Standards.
2. A sterile cotton swab was dipped into this solution, excess was removed and a lawn plate was created
Day 5: Results
Only one sample showed multidrug resistance (Sample 5), but
that was more than enough to remind us why responsible antibiotic use matters
so much not only in people but animals too!
It’s a great reminder that even the smallest organisms can
teach us some pretty big lessons.
Comment below and tell us what you’d like to learn about next!
Click here to check out our updated map with our exciting results!
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