The Resistance is Out There. . .
Come collect some samples with us!
What's the scoop with AMR and public wastewater?
Antimicrobial
resistance (AMR) is one of the most pressing global health threats of our time.
It occurs when bacteria, like Escherichia coli, develop the ability to
survive treatments with antibiotics that would normally kill them, making common
infections harder to treat and increasing the risk of disease spread and severe
illness. In areas where urban, agricultural, and industrial
activities often intersect, runoff and floodwaters may serve as silent
reservoirs for antibiotic-resistant bacteria. In this project, we explore the
presence of AMR E. coli in water from various high-risk environments and
assess the potential exposure risks to nearby human populations through
antibiotic susceptibility testing.
Come
along with us!
Here’s what you’ll need:
Gloves
Sterile sample cups
Ziploc bags
Scooping pole
Cooler for samples
70% isopropyl alcohol
Permanent marker for labelling
Location Selection: Five locations were chosen due to their proximity to residential, industrial, and agricultural zones.
So, how did we do it?
We put on sterile gloves to ensure minimal contamination of our samples.
We then labeled a sterile sample cup and securely fitted it into the scooping pole, ensuring the lid was removed beforehand.
We lowered the scooping pole into the water source until the cup was filled to approximately 60 mL.
We then removed the sample cup from the scooping pole, replaced the cover, and stored it in a cooler at 4 degrees Celsius
Steps 1-4 were repeated at 4 other locations until a total of 5 samples were collected
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Join us next time as we head to the laboratory to process our samples!
What do you think will grow in our petri dishes?
Comment your guesses below!
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